Intracellular calcium variation analysis of follicular cells

Authors

  • A. M. Herrera - Navarro División de Estudios de Posgrado, Facultad de Ingeniería, Universidad Autónoma de Querétaro
  • I. R. Terol - Villalobos Centro de Investigación y Desarrollo Tecnológico en Electroquímica, Querétaro
  • H. Jiménez - Hernández Centro de Investigación e Ingeniería Industrial, Querétaro
  • H. Peregrina - Barreto Laboratorio de Investigación en Control Reconfigurable
  • J. J. González - Barbosa CICATA, Instituto Politécnico Nacional

Abstract

 

This paper presents a method for the measurement of the variation of intracellular calcium in follicular cells. This proposal consists of two stages: (i) The detection of the cell's nuclei, and (ii) the analysis of the fluorescence variations. The first stage is performed with the modified-watershed transformation, where the marker process is controlled by establishing own criterion. The detection process homogenizes the luminance conditions through a morphological filter and uses as descriptors the edges of the cells. In the second stage, the variations of fluorescence are associated with changes in intracellular Ca2+, which are modeled as an exponential decay function. Then, we present a new morphological process called medium reconstruction process, that smoothes the data in the process of model creation. This process uses the information of under and over model of the signal, through the properties of reconstruction operators, keeping the internal structure of the original signal. Finally, using amphibian cells in an experimental process, the results obtained are shown after applying the proposal to a group of cells.

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Published

2013-01-15

How to Cite

Herrera - Navarro, A. M., Terol - Villalobos, I. R., Jiménez - Hernández, H., Peregrina - Barreto, H., & González - Barbosa, J. J. (2013). Intracellular calcium variation analysis of follicular cells. Revista Mexicana De Ingenieria Biomedica, 34(1), 71–87. Retrieved from https://rmib.com.mx/index.php/rmib/article/view/205

Issue

Vol. 34 No. 1 (2013): January - April

Section

Research Articles

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