Principal Components by FTIR Spectroscopy as Innovative Characterization Technique during Differentiation of Pluripotent Stem Cells to Pancreatic Cells

  • M. M. Mata Miranda SEDENA, Centro Militar de Ciencias de la Salud, Escuela Médico Militar-Escuela Militar de Graduados de Sanidad. Instituto Politécnico Nacional, Centro de Investigación en Biotecnología Aplicada-Tlaxcala
  • C. I. Guerrero Robles Instituto Politécnico Nacional, Escuela Superior de Medicina
  • M. Rojas López Instituto Politécnico Nacional, Centro de Investigación en Biotecnología Aplicada-Tlaxcala
  • R. J. Delgado Macuil
  • C. A. González Díaz SEDENA, Centro Militar de Ciencias de la Salud, Escuela Médico Militar-Escuela Militar de Graduados de Sanidad. Instituto Politécnico Nacional, Escuela Superior de Medicina
  • V. Sánchez Monroy SEDENA, Centro Militar de Ciencias de la Salud, Escuela Médico Militar-Escuela Militar de Graduados de Sanidad. Instituto Politécnico Nacional, Escuela Nacional de Medicina y Homeopatía.
  • D. G. Pérez Ishiwara Instituto Politécnico Nacional, Escuela Nacional de Medicina y Homeopatía
  • G. J. Vázquez Zapien SEDENA, Centro Militar de Ciencias de la Salud, Escuela Médico Militar-Escuela Militar de Graduados de Sanidad. Instituto Politécnico Nacional, Centro de Investigación en Biotecnología Aplicada-Tlaxcala
Keywords: Pluripotent Stem Cells, Differentiation, FTIR Spectroscopy, Principal Components

Abstract

Two of the greatest challenges in Stem Cells (SCs) biology and regenerative medicine, are differentiation control of SCs and ensuring the purity of differentiated cells. In this sense, fast, efficient and accurate techniques for SCs characterization and their differentiation into different cell lineages are needed. The aim of this study was to analyse Pluripotent Stem Cells (PSCs) and Differentiated Pancreatic Cells (DPCs) by Fourier Transform Infrared (FTIR) spectroscopy and Principal Component Analysis (PCA). For this purpose, we differentiated PSCs toward DPCs, characterizing the differentiation process at different stages (0, 11, 17 and 21 days) through light microscopy and vibrational spectroscopy. FTIR spectra were analysed with the multivariate method of PCA, using the second derivatives in the protein, carbohydrate and ribose regions. The results indicate that the PCA allows to characterize and discriminate PSCs and DPCs at different stages of differentiation in the analysed spectral regions. From these results, we concluded that the PCA allows the chemically and structural characterization of PSCs and the different stages of their differentiation in a fast, accurate and non-invasive way.

Downloads

Download data is not yet available.
Published
2017-01-15
How to Cite
Mata Miranda, M. M., Guerrero Robles, C. I., Rojas López, M., Delgado Macuil, R. J., González Díaz, C. A., Sánchez Monroy, V., Pérez Ishiwara, D. G., & Vázquez Zapien, G. J. (2017). Principal Components by FTIR Spectroscopy as Innovative Characterization Technique during Differentiation of Pluripotent Stem Cells to Pancreatic Cells. Mexican Journal of Biomedical Engineering, 38(1), 225-234. Retrieved from https://rmib.com.mx/index.php/rmib/article/view/25
Section
Special Issue