Adhesion and proliferation of bone marrow mesenchymal stem cells ontopoli-Llactic acid (PLA) fiber scaffolds

  • M. V. Granados Laboratorio de Bioingeniería de Tejidos; DEPeI, Facultad de Odontología, UNAM 2 Facultad de Ciencias, UNAM.
  • J. J. Montesinos Montesinos Laboratorio de Células Troncales Mesenquimales, Unidad de Investigación de Oncología, Hospital de Oncología, Centro Médico Nacional, IMSS
  • M. A. Álvarez Pérez Laboratorio de Bioingeniería de Tejidos; DEPeI, Facultad de Odontología, UNAM
Keywords: Scaffolds, Mesenchymal Stem Cells, Air Jet Spinning, Biocompatibility

Abstract

Fiber scaffolds have received increasing interest as promising biomaterials for potential application in the field of tissue regeneration. In this sense, we optimized the parameters for the synthesis of different concentrations (6, 7, and 10 %) of poly-lactic acid (PLA) scaffolds by air jet spinning technology (AJS). The PLA scaffolds were characterized by Scanning Electron Microscopy (SEM) and Fourier Transform Infrared Spectroscopy (FTIR) analysis. Our results by SEM micrographs showed that scaffolds have a fibrilar morphology with nanoscale diameter of fibers. Biocompatibility assay was observed through an in vitro experiment based on cell attachment, MTT and cell-material interaction assay when culturing bone marrow-derived mesenchymal stem cells onto the PLA spun membrane scaffolds. Our data indicate that fiber membrane of PLA scaffold increase the cellular response, are not cytotoxic when compared to thin films of PLA. Thus; the proposed synthesis method has potential for easy processing of spun fibrilar scaffolds with good biocompatibility and could be a promising economical biomaterial with future potential applications in tissue regeneration.
Published
2017-01-15
How to Cite
Granados, M. V., Montesinos Montesinos, J. J., & Álvarez Pérez, M. A. (2017). Adhesion and proliferation of bone marrow mesenchymal stem cells ontopoli-Llactic acid (PLA) fiber scaffolds. Mexican Journal of Biomedical Engineering, 38(1), 288-296. Retrieved from https://rmib.com.mx/index.php/rmib/article/view/32
Section
Special Issue