Effect of dehydroepiandroesterone and triamcinolone acetonide on 3T3-L1 cell line

  • G. Gutiérrez Iglesias Departamento de Posgrado, Escuela Superior de Medicina, Instituto Politécnico Nacional
  • R. López Marure Instituto Nacional de Cardiología Ignacio Chávez
  • A. Parra Barrera Departamento de Posgrado, Escuela Superior de Medicina, Instituto Politécnico Nacional
  • A. J. Sotelo Camero
  • C. C. Calzada Mendoza Departamento de Posgrado, Escuela Superior de Medicina, Instituto Politécnico Nacional
  • J. I. León Pedroza Departamento de Posgrado, Escuela Superior de Medicina, Instituto Politécnico Nacional
Keywords: Triamcinolone acetonide, dehydroepiandroesterone, 3T3-L1 fibroblast, Keloids scars

Abstract

The skin in the human is the largest organ, his integrity represents protection against various chemical, biological and mechanical agents. The injuries in this tissue are solved by forming a scar, however, different molecular alterations may overstimulate this process, leading to the formation of aberrant scars (hypertrophic or keloid). The most recommended treatment for such injuries is the intralesional application of triamcinolone acetonide (TA) and on the other hand, dehydroepiandrosterone (DHEA) is a pro-hormone that has a wide variety of biological effects such as regulation of the synthesis of collagen fibers, cell protection, anti-tumor properties, anti-inflammatory and anti-oxidant. In this paper, the combination of AT-DHEA on proliferation and cell death in fibroblast cell line 3T3-L1 was studied. The results showed that the AT 100 and 1000 M DHEA to inhibit proliferation by 50 and 40% respectively. The combination of AT-DHEA (10000-10 M) inhibits cell proliferation and induce programmed cell death, so this combination could be used in hypertrophic or keloid scars for disposal.
Published
2017-01-15
How to Cite
Gutiérrez Iglesias, G., López Marure, R., Parra Barrera, A., Sotelo Camero, A. J., Calzada Mendoza, C. C., & León Pedroza, J. I. (2017). Effect of dehydroepiandroesterone and triamcinolone acetonide on 3T3-L1 cell line. Mexican Journal of Biomedical Engineering, 38(1), 297-305. Retrieved from https://rmib.com.mx/index.php/rmib/article/view/33
Section
Special Issue